丹翘液对脂多糖诱导RAW264.7细胞炎症相关因子的抑制效应分析

doi:10.11843/j.issn.0366-6964.2015.12.023

Abstract

Abstract:

The aim of the present study was to study the anti-inflammatory effects and possible underlying mechanisms of Danqiao liquid in LPS-stimulated RAW264.7 cells.The cytotoxicity of Danqiao liquid was detected by MTT method.The NO kit assay was adopted to detect the effect of Danqiao liquid on NO release from LPS-induced RAW264.7 cells.ELISA was used to evaluate the production of TNF-α，IL-6 and PGE₂ in LPS-induced RAW264.7 cells.Real-time PCR was used to detect the transcription of COX-2，iNOS，TNF-α and IL-6 in LPS-induced RAW264.7 cells.The protein expression of nuclear NF-κB p65 was detected by Western blot.The result showed that the safe medication range of Danqiao liquid was less than 700 μg•mL^-1.Compared with the LPS model group，Danqiao liquid(100，300，600 μg•mL^-1) could reduce the secretion of NO，PGE₂，TNF-α，and IL-6 in cells induced by LPS，and significantly inhibit the mRNA transcription of iNOS，COX-2，TNF-α，IL-6 and the protein expression of NF-κB p65.In conclusion，this study preliminarily proves the protective effect of Danqiao liquid on LPS-induced RAW264.7 macrophages.Its action mechanism may be related to inhibit NF-κB signal pathway and the genes expressions and secretion of inflammatory mediators and cytokines.

CLC Number:

S853.9

WEI Li-qin，WANG Dong-sheng，DONG Shu-wei，KUANG Xiao-jiao，ZHANG Shi-dong，YAN Zuo-ting. Inhibiting Effect Analysis of Danqiao Liquid on Inflammation-related Factors Induced by LPS in RAW264.7 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2299-2306.

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Inhibiting Effect Analysis of Danqiao Liquid on Inflammation-related Factors Induced by LPS in RAW264.7 Cells

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